Could the GMO produce any 'new' metabolites or toxins likely to have deleterious effects on parasites or predators? If so, elaborate.

C2 C6

What other unintended effects may result from the release? Please include consideration of the effect of the GMO on the community ecology at the release site.

C3,D4 , C8

Are any of the likely gains directly linked to losses in other characteristics of the organisms?

General Information

Will the GMOs in this release be allowed to breed? If not, is breeding planned for later releases or commercial use?

D2

Are the arrangements for handling any offspring the same as those for the experimental organisms? If not, please specify the arrangements.

D7

Can the changed or added genetic material be transmitted by means other than by reproduction normal for the species or to any other species? If so, specify, and elaborate its effects.

D2, D3

Do natural populations of the parental organism exist in Singapore (including water bodies such as reservoirs, rivers, lakes, or coastal waters)? If so, do the natural populations cause problems with other organisms? Specify the organisms and the problems.

A3, A5

If no natural populations of the organism to be modified exist in Singapore, could the modified characteristics enhance the ability of the species to establish populations in aquatic habitats?

C1, D1

Has any experimental work been done on phenotypic expression of the novel genetic material in naturally occurring organisms (e.g. cross-breeding of GMOs with wild/farmed stocks)? If so, what were the results?

A6, D2

What is the likelihood of the novel genetic material entering the gene pool of natural populations?

A6, D2

Could the entry of the novel genetic material into the gene pool of a natural organism have any effect on the distribution and abundance of the organism or on associated fisheries, the environment or public health? If so, please explain.

C8, D8, D4

What mechanisms will be used to prevent dispersal of the GMO into other ecosystems?

D7